Hybridoma, which produces anti hCG monoclonal antibody ( mAb), was employed in the double mouthed roller, in which growth and antibody yield of the cells were compared with those cultivated in T flask. 利用分泌抗人绒毛膜促性腺激素(hCG)单克隆抗体的小鼠杂交瘤细胞作为检验材料,对培养在双口滚动培养装置及常规T形瓶中的细胞生长和单抗分泌进行了比较。